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. 2018 May 8;9:968. doi: 10.3389/fimmu.2018.00968

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Copyright © 2018 Muñoz-Caro, Conejeros, Zhou, Pikhovych, Gärtner, Hermosilla, Kulke and Taubert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time dependency of Dirofilaria immitis-induced neutrophil extracellular traps. Canine polymorphonuclear neutrophils (PMN) were cocultured with microfilariae (A) or L3 (B) for 8 h. Sytox Orange-positive signals were analyzed every hour by spectrofluorometric analysis using an automated plate monochrome reader. (A) a: significant difference between PMN and 100 microfilariae; b: significant difference between PMN and 300 microfilariae. (B) a: significant difference between PMN and 10 L3; b: significant difference PMN and 20 L3. Abbreviation: ns, not significant.