Table 4.
Parameters used to assess morphological difference after 14 days in culture.
| 8-Well chamber | PEEK chamber | p-values | |
|---|---|---|---|
| Morphological measures | |||
| Myotube width (μm) | 14.32 ± 2.10 | 14.00 ± 2.75 | 0.9949 |
| Myotube length∗ (μm) | 172.10 ± 18.35 | 165.9 ± 11.82 | 0.9672 |
| Number of myotubes (au) | 25.00 ± 8.74 | 21.17 ± 8.23 | 0.8109 |
| Number of nuclei per myotube (au) | 6.818 ± 1.32 | 8.770 ± 2.03 | 0.1000 |
| Cell density (au) | 139.0 ± 26.45 | 124.6 ± 28.34 | 0.6611 |
| Fusion index (%) | 45.93 ± 6.94 | 52.87 ± 2.96 | >0.9999 |
| Distance between myotubes (μm) | 14.47 ± 5.55 | 8.63 ± 2.58 | 0.414 |
| Number of myotube branches (au) | 3.17 ± 1.83 | 2.32 ± 1.47 | 0.261 |
Mean measures of the myotube parameters and their units from their respective constructs (chamber type). Au, arbitrary units. ∗Due to limitation of the image frame myotube length measurements are possibly greater than that measured.