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. 2018 May 8;9:973. doi: 10.3389/fimmu.2018.00973

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Copyright © 2018 Moll, Walter, Rezende, Helfinger, Vasconez, De Oliveira, Greten, Olesch, Weigert, Radeke and Schröder.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NADPH oxidase expression in the colon. (A) Analysis of mRNA expression in murine colon tissue. Relative expression to housekeeping gene EF. Genes indicated are p22phox (p22), Duox2 (D2), Nox1 (N1), NoxA1 (NA1), NoxO1 (N01), Nox2 (N2) p67phox (p67), p47phox (p47), and Nox4 (N4). n = 7–8. (B) HEK293 cells overexpressing Nox1 were transfected with cytosolic subunits of the NADPH oxidase complex as indicated. Reactive oxygen species were measured with L012 (200 μmol/L). Activation of p47phox was triggered by phorbol myristate acetate (100 nmol/L). (C) In situ hybridization (RNAScope^® DAB staining) showing the expression of NADPH oxidase subunits NoxO1, p47phox, Nox1, and Nox2 in murine colon tissue. Nuclei were counterstained with hematoxylin. Scale bars indicate 100 μm. (D) Quantitative RT-PCR, RNAScpoe^®, and immunoblotting analysis of Nox1 protein expression and in colon tissue of wild-type (WT) and NoxO1 knockout (NoxO1^−/−), n = 6, *p < 0.05 WT vs. NoxO1^−/−.