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NoxO1-dependent reactive oxygen species (ROS) formation in colon crypts. A + B Crypts from colon (A) or small intestine (B) were isolated. ROS were measured by chemiluminescence with L-012 (200 μmol/L). Activation of p47phox was triggered by phorbol myristate acetate (PMA, 100 nmol/L). Superoxide anions were decomposed with superoxide dismutase (SOD, 300 U/mL); n = 4.
