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. 2018 May 8;9:973. doi: 10.3389/fimmu.2018.00973

Figure 5.

Figure 5

NoxO1 has a protective role in dextran sulfate sodium (DSS)-induced colitis and DSS/azoxymethane (AOM) colon carcinoma. (A) In situ hybridization (RNAScope®) showing the expression of Nox1, NoxO1, Nox2, and p47phox in murine colon tissue upon treatment with DSS. Nuclei were counterstained with hematoxylin, scale bars indicate 100 μm. (B) Quantification of histological damage and number of ulcers on day 8 of the colitis model. n = 7–8, *p < 0,05, Mann–Whitney exact test. Representative pictures of hematoxylin and eosin (H&E) staining for wild-type (WT) and NoxO1−/− are shown. Scale bar indicates 200 μm. (C) Immunohistochemistry staining of macrophage marker F4/80. Representative pictures and analysis of F4/80-positive staining per nuclei. n = 7–8, *p < 0.05. Scale bars indicate 500 μm. (D) Body weight relative to day 0 of WT and NoxO1−/− mice treated with AOM at day 0 and 3 cycles of DSS in drinking water. n = 9, *p < 0.05. (E) Tumor burden at day 70 of DSS/AOM-treated animals. n = 8–10.