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. 2018 May 8;9:533. doi: 10.3389/fimmu.2018.00533

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Copyright © 2018 Al-Mozaini, Tsolaki, Abdul-Aziz, Abozaid, Al-Ahdal, Pathan, Murugaiah, Makarov, Kaur, Sim, Kishore and Kouser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Multiplex cytokine analysis of supernatants collected at 24 and 48 h phagocytosis of Mycobacterium bovis BCG by THP-1 cells incubated with or without properdin and thrombospondin repeats (TSR) 4+5. The supernatants were collected from phagocytosis assay of M. bovis BCG in the presence or absence of properdin and TSR4+5 at 24 and 48 h time point. The levels of cytokine production were measured for (A) (IL-6, IL-10, IL-12p40, IL-12p70, IL-1α, IL-1β, TNF-α, IL-13, IL-15, IL-17A, IL-9, and TNF-β), (B) chemokines (MCP-3, MDC, Eotaxin, Fractalkine, GRO, IL-8, IP-10, MCP-1, and MIP-1α), (C) growth factors (IL-9, IL-2, EGF, FGF-2, G-CSF, GM-CSF, IL-3, IL-4, IL-5, IL-7, and VEGF), and (D) related ligands and receptors (IFN-α2, IFN-γ, FLT-3L, IL-1RA, and sCD40L) using multiplex analysis. Error bars represent SD. A one-way ANOVA test was performed on the data to determine significant differences in expression of cytokine production by properdin or TSR4+5. All comparisons were significant (p < 0.05), unless where shown (ns, not significant, p > 0.05). Supernatants were analyzed in duplicate.

Multiplex cytokine analysis of supernatants collected at 24 and 48 h phagocytosis of Mycobacterium bovis BCG by THP-1 cells incubated with or without properdin and thrombospondin repeats (TSR) 4+5. The supernatants were collected from phagocytosis assay of M. bovis BCG in the presence or absence of properdin and TSR4+5 at 24 and 48 h time point. The levels of cytokine production were measured for (A) (IL-6, IL-10, IL-12p40, IL-12p70, IL-1α, IL-1β, TNF-α, IL-13, IL-15, IL-17A, IL-9, and TNF-β), (B) chemokines (MCP-3, MDC, Eotaxin, Fractalkine, GRO, IL-8, IP-10, MCP-1, and MIP-1α), (C) growth factors (IL-9, IL-2, EGF, FGF-2, G-CSF, GM-CSF, IL-3, IL-4, IL-5, IL-7, and VEGF), and (D) related ligands and receptors (IFN-α2, IFN-γ, FLT-3L, IL-1RA, and sCD40L) using multiplex analysis. Error bars represent SD. A one-way ANOVA test was performed on the data to determine significant differences in expression of cytokine production by properdin or TSR4+5. All comparisons were significant (p < 0.05), unless where shown (ns, not significant, p > 0.05). Supernatants were analyzed in duplicate.