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. 2018 May 8;9:615. doi: 10.3389/fpls.2018.00615

FIGURE 4.

FIGURE 4

Tomato PUB13 works with group III E2s to ubiquitinate FLS2-KD. FLS2-KD was ubiquitinated in the presence of GST-SlPUB13 and a member of group III E2 in in vitro ubiquitination assay. The assay was performed with recombinant E1, E2, GST-SlPUB13, ubiquitin (Ub), and MBP-FLS2-KD. Reactions with non-group III E2 UBC27 were used as control. The MBP protein was used as control for the substrate. This experiment was repeated two times with similar result.