FIGURE 5.

Expression of LOX-1, MCP-1, and SR-BI in the arterial wall of sedentary (S) and trained (T) animals. Immunoblot was performed by utilizing 40 μg of total protein from the aortic arch of S and T animals after 48 h of the last exercise session in order to confirm results obtained by RT-qPCR. Immunoblot was performed by using anti-LOX-1 (1:100); anti-MCP-1 (1:1000), anti-SR-BI (1:1000) and anti-ABCA-1 (1:50; not visualized) primary antibodies and horseradish peroxidase-linked secondary antibody (see section Materials and Methods for more details). The band densities of the respective lanes, stained by Ponceau, were used for normalization. The results were expressed as arbitrary units, related to mean of the sedentary animals, which was set as 100. Comparisons were done by the unpaired Student t-test (mean ± SEM). Representative images (n = 4–8, as indicated).