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. 2018 May 8;9:492. doi: 10.3389/fphys.2018.00492

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Copyright © 2018 Deist and Lamont.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Principal component analysis shows clear clustering by tissue type. Each dot represents a tissue from an individual chicken. Principal component 1 (PC1) separated the Harderian gland samples (green) from the other immune tissues. Principal component 2 (PC2) separated the Bursa (pink), Thymus (purple), and Spleen (blue). Ellipses were drawn with a 95% confidence. Dots with a black center were from Experiment 2. This plot was generated using pcaExplorer.