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. 2018 May 8;9:908. doi: 10.3389/fmicb.2018.00908

FIGURE 1.

FIGURE 1

Generation and characterization of hiPSC-HLCs. (A) The three-step hepatic differentiation includes endoderm priming (days 0–3), hepatic specification (days 4–10), and maturation (days 11–14). Cell morphology at days 0, 4, 10, and 14 is also shown (bar = 100 μm). (B) Fold changes of human hepatic specific gene transcriptions during the hepatic differentiation by qRT-PCR (n = 6). The hGAPDH gene served as the control. The primers were as shown in Supplementary Table 2. (C) Immunocytochemistry (ICC) to visualize the hepatic specific proteins hALB, hNTCP, hRXR, and hHNF4α in differentiated hiPSC-HLCs (bar = 20 μm). (D) Representative FACS dot plots for positive ratios of hALB, hNTCP, hRXR, and hHNF4α in mature hiPSC-HLCs and in hiPSC (as negative control). (E) Statistics for different batches of hiPSC-HLCs (n = 6). (F) The levels of hALB and hAAT in culture supernatant of differentiated hiPSC-HLCs (n = 6). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.