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. 2018 May 8;12:121. doi: 10.3389/fncel.2018.00121

FIGURE 6.

FIGURE 6

Accumulation of b-CTF in J20 mice. hAPP processing results in intracellular fragment b-CTF, the Aβ peptide and secondary products (A). Detection of hAPP gene by gel electrophoresis on a 1.5% agarose gel. 50- to 1,000-bp size markers; lane 1, J20-TG positive; lane 2, J20 Non-TG; lane 3, pcr ctl; lane 4 J20-PV-Cre and lane 5, J20-TG p120 (B). Levels of b-CTF at 30 and 120 days (C). Data are expressed as mean (± standard error of the mean) percentage of media control b b-CTF levels. (C) Both p30 J20-TG, and p30 J20-PV showed significant levels of b-CTF as detected in a sandwich ELISA (IBL, hAPP b-CTF Assay Kit, Japan). At p120 J20-TG mice showed more that 50% increase in b-CTF (C). Neither of the control had any significant b-CTF levels (C), p < 0.005, data are expressed as mean (± standard error of the mean) percentage of media control b-CTF levels. ∗p < 0.005 and NS p > 0.05.