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. 2018 Mar 29;15(6):4798–4804. doi: 10.3892/etm.2018.6007

Figure 1.

Figure 1.

Treatment with TMZ inhibits proliferation, and induces apoptosis and autophagy in U251 cells. (A) MTT assay was performed to examine the proliferation of U251 cells treated with TMZ (1–30 µg/ml). *P<0.05 vs. control. &P<0.05 vs. TMZ (1 µg/ml). (B) Flow cytometry was performed to examine the apoptosis of U251 cells treated with TMZ (1–30 µg/ml). *P<0.05 vs. control. #P<0.05 vs. TMZ (5 µg/ml). &P<0.05 vs. TMZ (10 µg/ml). (C) Western blot analysis was conducted to determine the levels of autophagy-related proteins in U251 cells treated with TMZ (30 µg/ml). *P<0.05 vs. control. (D) The ratio of LC3-II to LC3-I was calculated in U251 cells treated with TMZ (30 µg/ml). *P<0.05 vs. control. Non-treated U251 cells were used as the control in each assay. TMZ, temozolomide.