Table 1.

Primer/probe sequences used for determination of nitric oxide synthase isoform expression

NOS isoform	Primer/probe
eNOS	Forward: TCTGCGGCGATGTCACTATG
	Reverse: CATGCCGCCCTCTGTTG
	Probe: CCAGCGTCCTGCAAACCGTGC
iNOS	Forward: TGACGCCAAACATGACTTCAG
	Reverse: GCCATCGGGCATCTGGTA
	Probe: AATTCACAGCTCAATCCGGTACGCTGG
nNOS	Forward: GACTGATGGCAAGCATGACTTC
	Reverse: GCCCAAGGTAGAGCCATCTG
	Probe: TGGAACTCGCAGCTCATCCGCTATG

eNOS = endothelial nitric oxide synthase; iNOS = inducible nitric oxide synthase; nNOS = neuronal nitric oxide synthase; NOS = nitric oxide synthase.