Figure 1.

Expression of miR-155 in the BV2 cells, primary microglia and the mouse cortex. miR-155 expression was measured in (A) BV2 and (B) primary microglia following treatment with 100 ng/ml LPS alone or 10 ng/ml LPS and then 100 ng/ml LPS for 2 or 6 h. (C) miR-155 expression in the cortex was also measured. Tissue from the cortices of inflammatory and endotoxin tolerant mice were extracted at the indicated times (1, 3 and 7 days). miR-155 expression was measured using reverse transcription-quantitative polymerase chain reaction. Experiments were performed at least three biologically independent replicates and data are presented as the mean ± standard error of the mean. *P<0.05. miR, microRNA; LPS, lipopolysaccharide.