Figure 2.

Reverse transcription-quantitative polymerase chain reaction and western blotting analysis of chloride intracellular channel 1 (CLIC1) expression levels in four types of epithelial ovarian cancer tissues and paired healthy ovarian tissues. (A) Relative CLIC1 mRNA level in ovarian cancer samples was higher than that in matched healthy samples. There were no differences between different histological tumor types. The mRNA levels were calculated by 2^−ΔΔCq method. Human β-actin was used as the internal control. Data are presented as mean ± SD. *P<0.01. (B) Representative images of western blotting from 4 groups of cancer and healthy samples are shown. Human β-actin was used as a loading control. (C) Signal intensities of the western blot bands were analyzed using Image J 1.49v. There was no difference in β-actin between healthy and tumorous tissues. CLIC1 protein level was significantly higher in tumor tissues than in healthy controls. *P<0.01.