FIG 5.

The lethality of CV-A6 correlates with viral replication and release. Shown are one-step growth curves of CV-A6 Changchun046 and Changchun098. Similar amounts of Changchun046 and Changchun098 viruses, based on genome quantification by qRT-PCR, were used to infect RD cells. After adsorbing the virus for 6 h, the infected cells were washed, the medium was replaced with maintenance medium, and the levels of released viruses in the medium were determined through qRT-PCR at 2-h intervals, starting 8 h postadsorption. The values are shown as means ± SD.