TABLE 1.

Reverse transcription, PCR, and sequencing primers used in this study

Primer	Sequencea (5′ to 3′)	Location	Region	Reference
AN32	GTYTGCCA	3009–3002b	VP1	46
AN33	GAYTGCCA	3009–3002b	VP1	46
AN34	CCRTCRTA	3111–3104b	VP1	46
AN35	RCTYTGCCA	3009–3002b	VP1	46
224	GCIATGYTIGGIACICAYRT	1977–1996b	VP3	46
222	CICCIGGIGGIAYRWACAT	2969–2951b	VP1	46
AN89	CCAGCACTGACAGCAGYNGARAYNGG	2602–2627b	VP1	46
AN88	TACTGGACCACCTGGNGGNAYRWACAT	2977–2951b	VP1	46
FL-F-1C	TTAAAACAGCCTGTGGGTTG	1–20c	5′ UTR	19
panEV-F	CCCCTGAATGCGGCTAATC	458–476c	5′ UTR	19
panEV-R	GCTGCTTATGGTGACAATC	629–610c	5′ UTR	19
5′UTR-EV-F-FW2	CCCTCTTAGTGGCTGCAATC	1314–1333c	VP2	19
VP1-222-FW2-R	TGGGGCAATAGTTAGTGTGA	1678–1659c	VP2	19
2715-R	TTCACCTCCACAACYCCTACYAGC	2715–2692c	VP1	19
3311-F	ATAACTAACACTGCAACCGACC	3272–329c	VP1	19
3441-R	GCCCARTCATTATGAGTGGC	3441–3422c	2A	19
4069-R	GCMTCCATCYTAGGTATCCCTA	4098–4077c	2B	19
4613-F	GTGGTCACAGTYATGGACGATC	4613–4634c	2C	19
5477-R	GTYGCCGTRCGGAGRATRGGTT	5406–5385c	3B	19
6150-F	GCAGGCRTTGTTCTCYAAGTAT	6127–6148c	3D	19
5332-F	GTTTTCCCAGTCACGACTTYCARGGBGCKTA	6785–6815c	3D	19
650-R	GTCACTGGATGGCCAATCC	632–650c	5′ UTR	This study
5331-F	CTCTTTGCTGGGTTCCAAG	5331–5349c	3A	This study
7434-R	GCTATTCTGGTTATAACAAATTTACC	7409–7434c	3′ UTR	This study

^aI, inosine; K = T + G, M = A + C, R = A + G, W = T + A, Y = C + T, B = C + G + T, V = A + G + C, N = A + T + C + G.

^bThe locations of all primers are relative to the genome of PV1 Mahoney (GenBank accession number J02281).

^cNumbering is based on coxsackievirus A6 strain Gdula (accession no. AY421764).