FIG 2.

Identification of the PLRV DRTE responsible for RTP translation. (A) Schematic representation of the wild-type PLRV subgenomic RNA1 ORFs and the deletion or insertion mutations that were used to define the DRTE regulating readthrough. Detection of RTP translation based on the Western blots represented in panel C is indicated to the right of the schematic. (B) Accumulation of PLRV antigen, measured by DAS-ELISA, in N. benthamiana leaves 3 and 4 days after agroinfiltration with wild-type virus (WT) or the RTD deletion mutants. Healthy controls were agroinfiltrated with bacteria that do not contain the PLRV genome insert. H, noninfiltrated plant leaves. (C) Western blot analysis of PLRV proteins in N. benthamiana tissue 3 to 5 days following agroinoculation with wild-type PLRV or the RTP mutants. Relative readthrough (Rel. RTP/CP) was calculated as the RTP/CP ratio, with that for wild-type PLRV set as 100%. Values represent the means (± standard error) determined from three independent experiments.