FIG 3.

Stem-loop RNA structure of the distal element is required for RTP translation. (A) Predicted RNA secondary structure of the PLRV distal element and the mutants constructed for detecting RTP translation. (B) Western blot analysis of the role of stem-loop 1 in mediating RTP translation. (C) Western blot analysis of role of stem loops 3 and 4 in mediating RTP translation and the functional effects of the size and position of stem loop 1. Relative readthrough (Rel. RTP/CP) was calculated as the RTP/CP ratio, with that for wild-type PLRV set as 100%. Values represent the means (± standard error) determined from three independent experiments.