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. 2018 May 14;92(11):e02146-17. doi: 10.1128/JVI.02146-17

FIG 2.

FIG 2

Flow cytometry gating strategy to analyze educated and noneducated NK cells. (A) Top, gating on lymphocytes, single cells, and CD3− CD56dim NK cells. Bottom, gating on NKG2A+/−, KIR2DL1+/−, KIR2DL2-3+/−, and KIR3DL1+/− cells within the NK cell population. (B) Gating on noneducated NK cells for two representative donors (donor 01, NKG2A− KIR2DL1− KIR2DL2-3− KIR3DL1− NK cells; donor 03, NKG2A− KIR2DL1− KIR2DL2-3− NK cells). (C) Boolean gating was used to identify educated NK cells by subtracting noneducated NK cells from total NK cells. The FACS plots depict NKG2A, KIR2DL1, KIR2DL2-3, and KIR3DL1 expression in noneducated (pink) and educated (black) NK cells for a donor carrying the HLA-E, HLA-Bw4, HLA-C2, and HLA-C1 ligands.