FIG 6.

Contribution of educated NK cells to ADCC against gp120-coated CEM.NKr-CCR5 cells. PBMC were stained with a donor-dependent combination of PE-conjugated antibodies to enable the separation of PBMC into a population containing educated NK cells (Educated⁺) and a population lacking educated NK cells (Educated⁻) using FACS. (A) Gating strategy used to define and collect whole PBMC (unstained PBMC), Educated⁺ PBMC (PE-positive gated lymphocytes), and Educated⁻ PBMC (PE-negative gated lymphocytes). (B) The levels of anti-HIV-1 ADCC mediated by whole, Educated⁺, and Educated⁻ PBMC against gp120-coated CEM.NKr-CCR5 target cells were assessed using the LDH cytotoxicity assay. Data were compared using the Friedman test followed by Dunn's post hoc test. A P value of <0.05 was considered significant.