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. 2018 May 14;92(11):e00110-18. doi: 10.1128/JVI.00110-18

TABLE 2.

Primers and probe for real-time PCR

Oligonucleotide Sequence (5′-3′) Positions (nt)a
NP_forward AAGCGACGTTCCAAGAACTG 1542–1561
NP_reverse GGGACTGCAACAGAACCATC 1716–1697
NP_probe FAM-TGCTCCGGCACCTTGCCTTCC-TAMRA 1647–1627
a

Numbering is from the sequence of the D/swine/Oklahoma/1334/2011 NP gene (accession number JQ922306). nt, nucleotides.