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Primers and probe for real-time PCR
| Oligonucleotide | Sequence (5′-3′) | Positions (nt)a |
|---|---|---|
| NP_forward | AAGCGACGTTCCAAGAACTG | 1542–1561 |
| NP_reverse | GGGACTGCAACAGAACCATC | 1716–1697 |
| NP_probe | FAM-TGCTCCGGCACCTTGCCTTCC-TAMRA | 1647–1627 |
Numbering is from the sequence of the D/swine/Oklahoma/1334/2011 NP gene (accession number JQ922306). nt, nucleotides.
