FIG 1.

Interaction between L and Z proteins of OW mammarenaviruses LCMV and LASV. 293T cells were transfected with a plasmid expressing Strep-tagged eGFP or the Z protein together with a plasmid expressing FLAG-tagged L protein. Plus and minus signs indicate plasmid presence and absence in the transfection mix. Plasmids for LCMV and LASV were used for panels A and B, respectively. An empty vector, pCAGGS, was used to identify nonspecific protein bands in Western blots. At 72 h posttransfection, cells were lysed and PD was performed using Strep-Tactin. Protein levels in clarified whole-cell lysate (Input) and eluate (PD: Strep) were examined by Western blotting. Numbers at the bottoms of anti-FLAG Western blots correspond to relative signal intensity of FLAG-tagged LCMV- or LASV-L protein in whole-cell lysate (Input) [L signal (%)]. The signal intensity of the L protein coexpressed with eGFP-Strep was set to 100%.