FIG 6.

Neither the proteasome nor the lysosome pathway is involved in degradation of the Z mutants in the presence of an active vRNP. Effect of the proteasome inhibitor MG132 (A) or the lysosome inhibitor ammonium chloride (NH₄Cl) (B) on Z's stability in the presence of an active vRNP. 293T cells were transfected with plasmids required for formation of active vRNP (pT7-MG/eGFP [MG/eGFP], pC-T7pol [T7 pol], pC-L [L], and pC-NP-HA [NP-HA]) together with or without a plasmid expressing the WT or the indicated mutant Z proteins. MG132 (A) and NH₄Cl (B) were added to culture media at 19 h or 5 h, respectively, posttransfection. At 48 h posttransfection, cells were harvested and Z protein expression levels in the S and P fractions were examined by Western blotting.