FIG 6.

Transcriptomic analysis of CD8⁺ T cells from unvaccinated mice with or without NK cell depletion following LCMV Cl-13 challenge. (A) Heat map representation of the GSEA-normalized enrichment scores of selected pathways with increased or decreased expression levels in NK cell-depleted animals compared to isotype control-treated animals at day 8 postchallenge (four mice per group). PI3K, phosphatidylinositol 3-kinase; CTLA-4, cytotoxic-T lymphocyte-associated antigen 4. (B) Gene interaction networks showing the leading genes with increased and decreased expression levels in unvaccinated animals following NK cell depletion. Networks were generated by using the Cytoscape DyNet plug-in, and interactions were inferred from GeneMania. (C) Expression of PD-1 on GP33-specfic CD8⁺ T cells in unvaccinated animals (n = 8 per group). (D) Scaled representation showing the log₂ fold changes in gene expression levels for modules associated with T cell and immune activation at day 8 post-LCMV challenge, comparing vaccinated and unvaccinated animals following NK cell depletion. All genes shown are significant, with a corrected P value cutoff of 0.05.