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. 2018 May 14;92(11):e02106-17. doi: 10.1128/JVI.02106-17

FIG 7.

FIG 7

FPC-HPV infection occurs independently of HSPGs, and particles show reduced binding affinity to heparin. (A) HPV16 (black) or FPC-HPV16 (white) infection of NaClO3-treated HeLa or HaCaT cells in the seed-over setup (as described for Fig. 3B). Infectious values are shown in percentages ± SD relative to untreated cell samples for all experiments. (B) Heparin binding affinity of HPV16 (black) and FPC-HPV16 (gray) was analyzed using HiTrap heparin HP columns (GE) and subsequent elution by increasing salt concentrations (300 mM to 1,500 mM). Depicted is a representative Western blot for HPV16 L1 of the eluted fractions 1 to 11 of HPV16 (upper panel) or FPC-HPV16 (lower panel) during salt gradient elution. Representative chromatograms of the HPLC runs were aligned to the corresponding elution fractions (HPV16, black line; FPC-HPV16, gray line). Salt concentrations corresponding to the main elution peak from at least 3 independent experiments are summarized in the table.