FIG 8.

The accessibility of the secondary receptor for virus interaction is limited. (A) HeLa or HaCaT cells were treated with 50 mM NaClO₃ for 2 days and subsequently infected with HPV16 (black) or FPC-HPV16 (white) in add-on experiments (as described for Fig. 3A). Relative infection values were normalized to 48-h infection samples of untreated cells in percentages ± SD. FACS, fluorescence-activated cell sorting. (B) AF488-labeled HPV16 (gray/green, upper panels) or FPC-HPV16 (gray/green, lower panels) particles were bound to untreated or NaClO₃-treated HeLa cells. Cells were fixed and stained for F-actin with Atto647N-phalloidin (red). Depicted are representative maximum intensity projections of confocal image stacks from at least three independent experiments. Virus signals were quantified relative to cell area for more than 150 cells per condition. (C) As described for panel B, using HaCaT cells.