FIG 1.

Neutralization properties of sCD300lf against murine norovirus virions. (A) Serially diluted sCD300lf was added to CW1 virions and inhibition of attachment was measured. The IC₅₀s were 0.2 μg/ml and 0.4 μg/ml for the plaque and viral attachment assays, respectively (error bars show SDs; three independent experiments were performed). These findings indicated that the sCD300lf was likely functional and properly folded. (B) A direct ELISA showed that sCD300lf was unable to bind the murine norovirus CR10 P domain or CW1 virions, whereas a positive control murine norovirus-specific nanobody (Nano-45) was able to detect the CR10 P domain and CW1 virions.