Skip to main content
. 2018 May 14;92(11):e00425-18. doi: 10.1128/JVI.00425-18

FIG 4.

FIG 4

HAX-1 inhibits virus replication in a PB1-F2-dependent manner. (a) Western blot showing the efficiency of HAX-1 knockout and HAX-1 overexpression in the cells used for the growth curve. (b and c) Different A549 cell lines were infected at an MOI of 0.01 with either VN/1203 wt, VN/1203 ΔF2, PR/8 wt, or PR/8 ΔF2 virus with TPCK-treated trypsin at 0.1 μg/ml. Supernatants were collected at the indicated time point postinfection, and virus titer was assessed by plaque assay on MDCK cells. Bars represent means (n = 3), with error bars indicating SDs. Fold changes between wt and ΔF2 viruses are indicated in the table below each graph. Student t test was used to compare virus titer at each time point. n.s., nonsignificant; n.d., not detected. *, P < 0.05; ***, P < 0.001. (d) Different A549 cell lines were infected at an MOI of 1 with either VN/1203 wt, VN/1203 ΔF2, PR/8 wt, or PR/8 ΔF2 virus. At the indicated time point postinfection, cells were lysed and processed through WB.