FIG 1.
Comparison of MAb binding profiles of WT and ΔHVR1 gpE1/gpE2 antigens. Purified recombinant WT gpE1/gpE2 (WT) or gpE1/gpE2 without the HVR1 domain of gpE2 (ΔHVR1) was immobilized on ELISA plates coated with GNA-lectin. Unbound protein was removed, followed by incubation of increasing amounts of the indicated cross-neutralizing MAb. The binding of these antibodies to the recombinant protein was detected by using anti-human or anti-mouse antibody. B6 is an irrelevant human immunoglobulin control. O.D.450, optical density at 450 nm.