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. 2018 May 14;92(11):e02141-17. doi: 10.1128/JVI.02141-17

FIG 2.

FIG 2

Quantitation of anti-gpE1/gpE2 (A) and antipeptide (residues 384 to 417) (B) antibodies after immunization of mice. (A) Purified recombinant gpE1/gpE2 with or without the HVR1 domain was immobilized on an ELISA plate coated with GNA. Heat-inactivated final-bleed sera from three different groups of mice were used at the indicated concentrations (control [C], gpE1/gpE2 [WT], or gpE1/gpE2 without the HVR1 domain [ΔHVR1]). Comparisons of antibody reactivity to gpE1/gpE2 with and without HVR1 at each matched concentration of sera showed no significant difference. Thus, WT and ΔHVR1 gpE1/gpE2-immunized mice showed similar titers for glycoprotein-reactive antisera. (B) An ELISA plate was coated with a peptide corresponding to residues 384 to 417 of HCV gpE2. Sera from immunized mouse groups were added at the indicated concentrations. The binding of antibody was detected with anti-mouse secondary antibody. Statistical analysis was done by one-way analysis of variance and a Tukey post hoc test (GraphPad); only statistically significant differences are highlighted. *, P < 0.05.