FIG 6.

Measurement of the effect of CTCF knockdown on HSV-1 reactivation in the rabbit eye. The eyes of rabbits latently infected with HSV-1 were swabbed for 10 consecutive days following treatment with either the AAV8-GFP (control) or AAV8-siCTCF, starting at day 28 p.i. Swabs were assayed by plaque assays for infectious virus. Additional swabs were obtained and plaque assayed starting on day 56 p.i. to determine whether rabbits continued to shed infectious virus in the siCTCF-treated group long term. An exact version of chi-square analysis for all pairwise comparisons with control (AAV8-GFP) was performed and indicated a significant increase in infectious virus present in the AAV8-siCTCF-treated animals versus the control group (P < 0.0001).