FIG 8.

LAT and lytic transcript relative abundance was determined from TG harvested at day 92 p.i. RNA was isolated and analyzed by qRT-PCR using primers and probes specific for the LAT intron, ICP0, HSV-1 DNA polymerase, and VP16 (see Table 3). The relative quantities were determined for viral genes and then normalized to the relative quantities of rabbit GAPDH to account for variations between samples. The average normalized values for each gene region are presented, along with the standard deviations. All samples have an n = 5 TG. One-way ANOVA showed that a significant increase in ICP0 transcription was found in siCTCF-treated rabbits versus the control animals.