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. 2018 May 14;92(11):e00173-18. doi: 10.1128/JVI.00173-18

TABLE 3.

Primers and probes

Target Sequence (5′-3′) GenBank accession no. (nucleotide range)
Rabbit GAPDH
    Primer, forward GCA CCA CCA ACT GCT TAG C
    Primer, reverse CCT CCA CAA TGC CGA AGT G
    Probe CTG GCC AAG GTC ATC C
HSV-1 ICP0 NC_001806 (121385–121453)
    Primer, forward GGC CGA GGG AGG TTT CC
    Primer, reverse CCG CTT CCG CCT CCT C
    Probe CTC CCA GGG CAC CGA C
HSV-1 VP16 NC_001806 (103946–104191)
    Primer, forward CCT CGA TGG TAG ACC CGT AA
    Primer, reverse ACA TTC GCG AGC ACC TTA AC
    Probe CAT AAA GTA CCC AGA GGC
HSV-1 DNA polymerase NC_001806 (65880–65953)
    Primer, forward AGA GGG ACA TCC AGG ACT TTG T
    Primer, reverse CAG GCG CTT GTT GGT GTA C
    Probe ACC GCC GAA CTG AGC A
HSV-1 LAT intron
    Primer, forward ACC CAC GTA CTC CAA GAA GGC NC_001806 (119721–119795)
    Primer, reverse TAA GAC CCA AGC ATA GAG AGC CA
    Probe TCC CAC CCC GCC TGT GTT TTT