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. 2018 May 15;1(1):15–27.

Figure 2.

Figure 2

In vitro transcription competition. Various molar ratios (competitor to transcription template) of the indicated LTR or nonspecific fragments were used to compete for transcription from a constant amount of the LTR transcription template (Nco I linearized p5′LTR-CAT). The transcription template produces the 633 nucleotide run-off transcript indicated. A. Competitors used (see also Figure 1): the intact LTR competitor, Acc I to Hind III; the fragments 1 through 5 formed by cleavage of the Acc I to Hind III fragment with Hae III; the nonspecific competitor made up of 593 bp of CAT gene sequences. The lane marked ALL shows the competition produced by the indicated molar excesses of all of the Hae III fragments mixed together in the reaction. B. Competitors which overlapped the Hae III sites; the Acc I to Ava I fragment which approximately represents the sequences of the 5′ half of the LTR; the Ava I to Hind III fragment which approximately represents the sequences of the 3′ half of the LTR; and the Sau3A I to Sau3A I fragment (all shown in Figure 1).