Table 3.

The biosensors and specific traits for the detection of AHLs produced by marine Vibrio.

Microbiosensors	Plasmid	Sensing system	AHL types	Report system	Functional expression	References
						author, year
C. violaceum CV026	—	CviI/RC	C6-HSL*; C4-HSL; C8-HSL; 3-oxo-C4−8-HSL	endogenous pigment	purple colony	McClean et al., 1997
E. coli	pSB 536	AhyI/R	C4-HSL*	LuxCDABE	bioluminescence	Tait et al., 2010
E. coli	pSB 401	LuxI/R	3-oxo-C6-HSL*; C6-HSL; C8-HSL	LuxCDABE	bioluminescence	Tait et al., 2010
E. coli MT102	pSB403	LuxI/R	3-oxo-C6-HSL*; C6-HSL; C8-HSL; 3-oxo-C8-HSL	LuxCDABE	bioluminescence	Charlesworth et al., 2015
E. coli	pSB 1075	LasI/R	3-oxo-C12-HSL*; C12-HSL; 3-oxo-C10-HSL	LuxCDABE	bioluminescence	Tait et al., 2010
A. tumefaciens NTL1	pDCI41E33	TraI/R	3-oxo-C4-HSL - 3-oxo-C12-HSL*; C6-HSL – C12-HSL	lacZ	β-galactosidase expression	O'Connor et al., 2015
A. tumefaciens NTL4	pZLR4	TraI/R	3-oxo-C8-HSL*; C6−14-HSL; 3-OH-C6−10-HSL; 3-oxo-C4−14-HSL	lacZ	β-galactosidase expression	Kumar et al., 2016
A. tumefaciens KYC55	pJZ372 pJZ384 pJZ410	TraI/R T7 T7	3-oxo-C8-HSL*; C6−10-HSL; 3-OH-C6−10-HSL; 3-oxo-C6-HSL; 3-oxo-C12-HSL	lacZ	β-galactosidase expression	Joelsson and Zhu, 2006
P. putida F117	pAS-C8	CepI/R	C8-HSL*; C10-HSL	gfp	green fluorescence	Steidle et al., 2001
P. putida F117	pKR-C12	LasI/R	3-oxo-C12-HSL*; 3-oxo-C10-HSL	gfp	green fluorescence	Krick et al., 2007

^*most sensitive AHLs.