Fig. 5.

BMC-MGC hybrids undergo reprogramming and can differentiate into CALR⁺ cells in the long term. (a) RT-PCR analysis of YFP⁺ hybrids FACS-sorted from NMDA-damaged retinae of GFAP-Cre/BM^R26Y mice, 24 hpi. Transcript levels of are expressed as fold-changes relative to hybrids-depleted retinae (NMDA retina). Values represent means ± S.E.M. (n ≥ 3). Statistical analysis is based on unpaired Student's t-test (Oct4, p = 0.3266^ns; SSEA1, p = 0.3205^ns, Sox2, p = 0.0139^⁎; Pax, p = 0.0153^⁎; Nestin, p = 0.2728^ns; Ascl1, p = 0.3358^ns; Six3, p = 0.3822^ns; Prox1, p = 0.2247^ns; Chx10, p = 0.2774^ns; Math5, p = 0.7969^ns; Math3, p = 0.0048^⁎⁎; CyclinD1, p = .0237^⁎). (b) Representative immunostaining images of retinal flat mounts from undamaged (CTR) and damaged (NMDA) GFAP-Cre/BM^R26Y chimeric mice, 3 wpi (n = 3). Yellow arrows show co-localization of YFP⁺ hybrids (green) with CALR (red). Scale bar: 20 μm. (c) Percentages of YFP⁺/CALR⁺ hybrids relative to total of YFP⁺ hybrids counted in random fields of retinal flat mounts from GFAP-Cre/BM^R26Y chimeric mice, 3 wpi (n = 3).