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. 2018 May 13;17:1533034618770305. doi: 10.1177/1533034618770305

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Sinomenine promotes G1-phase cell cycle arrest and apoptosis. (A B) U87 and U251 cells were treated with or without 16 μmol/L of sinomenine for 48 hours and examined for cell cycle distribution by flow cytometry after PI staining. The sub-G1 population was gated out, and the percentages of cells at the G0/G1, S, and G2/M phases were calculated. The statistic data in this figure represent the results from 3 independent experiments. (C), Apoptosis analysis by flow cytometry after annexin-V/PI staining. Top: representative dot plots of cells stained with annexin-V and PI. *P < .05 relative to vehicle-treated control cells. PI indicates propidium iodide.