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. 2018 May 13;17:1533034618770305. doi: 10.1177/1533034618770305

Figure 4.

Figure 4.

Restoration of SIRT1 rescues glioma cells from sinomenine-induced growth suppression. U87 cells were transfected with SIRT1-expressing plasmids or empty vector before treatment with 16 μM of sinomenine, and tested for proliferation (A), cell cycle distribution (B), and apoptosis (C). (A) BrdU incorporation assay. (B) Flow cytometric analysis of DNA contents after PI staining. (C) Apoptosis analysis after annexin-V/PI staining. *P < .05. SIRT1 indicates sirtuin 1; BrdU, bromodeoxyuridine; PI, propidium iodide.