Fig. 6.

Global transcriptome analysis after targeted knockdown for ASMER-1 and ASMER-2 in in vitro differentiated mature adipocyte. A. Venn-diagram of the overlapping genes affected by transfecting antisense oligonucleotides against ASMER-1 or ASMER-2 in in vitro differentiated hADSCs. B. Identification of upstream mediators that underlie the altered transcriptome after silencing of ASMER-1 or ASMER-2 through pathway enrichment analysis using Ingenuity Pathway Analysis (IPA). C and D. Significantly changed PPARG downstream genes identified by IPA after silencing of ASMER-1 (C) and ASMER-2 (D). Red and green highlight the up-regulated and down-regulated proteins, respectively, and the colour depth is correlated to the fold change. Orange and blue lines with arrows indicate indirect activation and inhibition, respectively. Yellow and grey dashed lines with arrows depict inconsistent effects and no prediction, respectively.