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. 2018 Mar 15;30:248–260. doi: 10.1016/j.ebiom.2018.03.010

Fig. 7.

Fig. 7

Functional characterization of the lncRNAs ASMER-1 and ASMER-2 in the adipogenesis. A. Micrographs of Oil-Red-O staining of the hADSC cell after differentiation at day 13 with silencing of ASMER-1 (two antisense oligonucleotides), ASMER-2 (two antisense oligonucleotides), Control and Mock at day 0 of differentiation. Scale bar is 300 μm. B. Lipid content was measured by quantification of Oil-Red-O staining (absorbance at 495 nm), n = 4, ***p < 0.001, **p < 0.01. C. hADSCs were induced to differentiate at day 0 after silencing of ASMER-1 (two antisense oligonucleotides). Gene expression of ASMER-1 as well as established genes regulated during adipogenesis (ADIPOQ, FABP4 and PPARG) were determined by Real-time PCR at different time points. n.s = not significant. D. After silencing of ASMER-2 (two antisense oligonucleotides) at day 0. Gene expression of ASMER-2 as well as established genes regulated during adipogenesis (ADIPOQ, FABP4, and PPARG) were determined by Real-time PCR at different time points. Silencing the expression of AMSER-2 resulted in significantly lower expression levels of 3 measured genes. n.s = not significant.