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. 2018 May 15;17:73. doi: 10.1186/s12934-018-0922-y

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Comparison between the budding indices obtained with ISM (black bars) and with manual counting (gray bars) with the respective standard deviations for the probe MM-Ho (a) and the probe MM 2.1 (b). The standard deviation of the manual counting was obtained from two mean values from a sample size of between 100 and 200 cells. The standard deviation of the automated cell detection is calculated as difference between values of a fit (spline function) and values of a capture event. Linear correlation between the manual and automatic recognition for the probe MM-Ho (c) and the probe MM 2.1 (d)