FIG 6.

B. subtilis ΔftsX or ΔftsE mutants show no difference in CXCL10 sensitivity from that shown by the B. subtilis ftsX⁺ ftsE⁺ parent strain. Sensitivities of ΔftsX (IU12981) and ΔftsE::Tn10 spec (IU12166) mutants to CXCL10 relative to the B. subtilis ftsX⁺ ftsE⁺ parent (IU12153) were determined using a fluorescence-based antimicrobial assay in NPB buffer (described in Materials and Methods). Each point represents the mean ± SEM of results of 4 independent experiments. Dose-response curves and IC₅₀s were obtained from duplicate wells from all independent experiments and fitted to a dose-response curve (log of inhibitor versus response-variable slope) using GraphPad Prism. See Table S3 for 95% CI values.