FIG 5.
Expression levels of crp and pla do not increase during Y. pestis ΔptsG infection. Cross sections of lungs from mice infected with Y. pestis ΔptsG carrying the pGEN-RFP and either the PtetO-GFP, Pcrp-GFP, or Ppla-GFP reporter construct were analyzed identically to those from the wild-type-infected counterparts. (A to C) Violin plots of relative gene expression levels in individual cells combined from the lung lesions of at least three mice at each time point, across two independent infection experiments measured at 48 and 72 hpi for Y. pestis ΔptsG expressing either the PtetO-GFP, Pcrp-GFP, or Ppla-GFP reporter construct, as indicated. Horizontal lines within the violins represent the 25th percentile, median, and 75th percentile. (D to F) Relative expression as a function of cell density for infection with Y. pestis ΔptsG carrying the PtetO-GFP, Pcrp-GFP, or Ppla-GFP reporter construct. Individual points represent individual cells analyzed from infections at 48 and 72 hpi.