TABLE 1.
Unsaturated fatty acid profile of the WT and S. aureus geh lip grown in the presence of human LDLs
| PG TC:TDBa | WT cultured in tryptone broth |
WT cultured in tryptone broth with human LDLs |
S. aureus
geh lip cultured in tryptone broth |
S. aureus
geh lip cultured in tryptone broth with human LDLs |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Normalized ion abundance/mg of cells | SD | Fatty acidsc | Normalized ion abundance/mg of cells | SD | Fatty acidsc | Normalized ion abundance/mg of cells | SD | Fatty acidsc | Normalized ion abundance/mg of cells | SD | Fatty acidsc | |
| 30:1 | 0.00 | 0.00 | NDb | 0.11 | 0.08 | ND | 0.13 | 0.06 | ND | 0.00 | 0.00 | ND |
| 31:1 | 1.96 | 0.19 | 16:1_15:0, 14:1_17:0, 12:1_19:0 | 7.46 | 0.38 | 16:1_15:0, 14:1_17:0, 12:1_19:0, 11:1_20:0 | 2.64 | 0.24 | 16:1_15:0, 14:1_17:0, 12:1_19:0 | 3.02 | 0.18 | 16:1_15:0, 14:1_17:0, 12:1_19:0, 11:1_20:0 |
| 32:1 | 1.49 | 0.07 | ND | 7.79 | 0.58 | 17:1_15:0, 16:1_16:0, 18:1_14:0 | 1.99 | 0.29 | 17:1_15:0, 15:1_17:0, 13:1_19:0 | 2.18 | 0.10 | 17:1_15:0, 16:1_16:0 |
| 33:1 | 12.76 | 1.01 | 18:1_15:0 | 214.05 | 19.82 | 18:1_15:0 | 14.19 | 0.59 | 18:1_15:0 | 28.02 | 1.43 | 18:1_15:0 |
| 34:1 | 0.00 | 0.00 | ND | 16.58 | 1.53 | 18:1_16:0, 16:1_18:0 | 0.00 | 0.00 | ND | 0.00 | 0.00 | ND |
| 34:2 | 0.00 | 0.00 | ND | 5.04 | 0.37 | 16:1_18:1, 18:2_16:0 | 0.00 | 0.00 | ND | 2.00 | 0.25 | 16:1_18:1, 18:2_16:0 |
| 35:1 | 17.07 | 1.75 | 20:1_15:0, 19:1_16:0 | 61.86 | 5.26 | 20:1_15:0, 18:1_17:0, 19:1_16:0 | 19.16 | 0.22 | 20:1_15:0, 19:1_16:0 | 14.66 | 0.46 | 20:1_15:0, 19:1_16:0, 18:1_17:0 |
| 35:2 | 0.00 | 0.00 | ND | 8.04 | 0.50 | 20:2_15:0, 18:2_17:0, 16:1_19:1 | 0.03 | 0.01 | ND | 6.04 | 0.23 | 20:2_15:0, 18:2_17:0 |
| 35:4 | 0.00 | 0.00 | ND | 1.46 | 0.26 | 20:4_15:0, 17:2_18:2, 20:3_15:1 | 0.00 | 0.00 | ND | 20.13 | 0.60 | 20:4_15:0 |
| 35:5 | 0.00 | 0.00 | ND | 0.00 | 0.00 | ND | 0.00 | 0.00 | ND | 1.27 | 0.15 | 20:5_15:0, 20:4_15:1 |
| 36:2 | 0.00 | 0.00 | ND | 46.15 | 4.14 | 18:1_18:1, 18:2_18:0 | 0.00 | 0.00 | ND | 0.39 | 0.16 | 18:2_18:0, 18:1_18:1 |
| 36:3 | 0.11 | 0.05 | ND | 17.05 | 1.56 | 18:1_18:2, 20:3_16:0 | 0.00 | 0.00 | ND | 0.20 | 0.10 | 18:2_18:1 |
| 36:4 | 0.00 | 0.00 | ND | 2.34 | 0.35 | 18:2_18:2, 16:0_20:4, 18:3_18:1 | 0.00 | 0.00 | ND | 0.25 | 0.06 | 18:2_18:2, 20:3_16:1, 20:4_16:0 |
| 37:2 | 0.00 | 0.00 | ND | 1.85 | 0.11 | 19:0_18:2 | 0.00 | 0.00 | ND | 0.38 | 0.15 | 19:0_18:2 |
| 37:4 | 0.00 | 0.00 | ND | 0.00 | 0.00 | ND | 0.00 | 0.00 | ND | 0.91 | 0.05 | 20:4_17:0 |
a
Detected as [M-H]− ions. TC, total chain length; TDB, total number of double bonds.
b
ND, not determined.
c
Fatty acids are listed in order of isomer abundance. An underscore between fatty acid designations indicates that each fatty acid may be present in either the SN1 or SN2 position, as tandem mass spectrometry data alone cannot rule out the possibility that lipid species exist as a mixture of positional isomers (85).