FIG 3.

Growth properties of T. kodakarensis host strain KU216 and three gene disruption strains. (A) Growth of the host KU216 strain (circles) and ΔTK1676 mutant (triangles) were examined in a synthetic amino acid medium without Na and Nm (minimal medium, ASW-AA-S⁰-Pyr-Ura-W). (B) Growth of ΔTK0218 mutant in the minimal medium and medium supplemented with Na or Nm are shown. (C) Growth of the ΔTK0218 ΔTK1650 mutant strain in the minimal medium and medium supplemented with Na or Nm. Open, filled, and gray symbols indicate ASW-AA-S⁰-Pyr-Ura-W, ASW-AA-S⁰-Pyr-Ura-W-Na(+), and ASW-AA-S⁰-Pyr-Ura-W-Nm(+) media, respectively. Each value is an average of those from three independent growth experiments. The vertical axis is represented on a logarithmic scale.