Supplementary Fig. 4.

MEG3 inhibits the CLU through directly binding to CLU protein. (A) MEG3 directly binds to CLU protein as the CLU antibody formed RNA-protein-antibody complexes suggested by EMSA assay; (B) The MEG3 spatial structure predicted by RNA structure (version 3.7). MEG3 was divided into four segments according to prediction, F1:1–389, f2: 390–731, F3: 732–1174, F4: 1175–1598; (C) C expression of LU mRNA and protein in SW1116 transfected with CLU siRNAs or not etermined by RT-PCR and western-blot (*P < .05); (D) Representative image of the trans-well assay to investigate migration ability (upper) and invasion ability (lower) of CRC cells with CLU down-regulation(si-CLU2), MEG3 over-expression (MEG3), MEG3 over-expression combinated with CLU over-expression (MEG3/CLU) or not (Blank).