Fig. 4.

SB suppresses pro-inflammatory cytokine expression in LPS-induced macrophages.

(A-B) Expression of TNF-α and IL-6 in SB- and LPS-treated RAW246.7 cells. RAW246.7 macrophages were stimulated with LPS for 6 h after pre-treating with SB for 1 h, and then the cells were collected with TRIzol. Gene expression of TNF-α and IL-6 was detected by qRT-PCR (n = 3). (C-D) Protein levels of TNF-α and IL-6 in the medium supernatant of SB- and LPS-treated RAW246.7 macrophages. RAW246.7 macrophages were pre-treated with SB for 1 h, and then stimulated with LPS for 12 h. The medium supernatant was collected and used for measuring the protein levels of TNF-α and IL-6 by ELISA (n = 3).