Table 2.
Nanostring and RT-qPCR significant comparisons. RT = RT-PCR, NS = NanoString Assay, n.s. = Not Significant. Nanostring and RT-PCR significant pValues are showed for each tested microRNA and comparison. P-Values are written in bold for those microRNAs that showed a significant differential expression in the corresponding comparison. Bold boxes frame those p-Values that were found significant with both technologies (RT & NS). All microRNAs were found up-regulated in significant comparisons. Comparison boxes that did not show any significant difference with both techniques were left empty.
| Nanostring and RT-qPCR Significant comparisons | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| p-Values |
||||||||||||
| miR-422a | miR-494-3p | miR-502-5p | miR-520f | miR-549a | miR-4443 | |||||||
| Comparisons | RT | NS | RT | NS | RT | NS | RT | NS | RT | NS | RT | NS |
| CTRL vs OLIGO | n.s. | 0.017 | n.s. | 0.014 | n.s. | 0.007 | ||||||
| CTRL vs ASTRO | n.s. | 0.022 | n.s. | 0.001 | n.s. | 0.001 | 0.045 | 0.0006 | ||||
| CTRL vs GBM | 0.005 | n.s. | 0.001 | n.s. | 0.045 | n.s. | 0.012 | 0.001 | 0.040 | 0.042 | 0.032 | n.s. |
| GBM vs OLIGO | 0.006 | 0.016 | 0.001 | 0.038 | 0.040 | 0.046 | 0.017 | 0.033 | n.s. | |||
| GBM vs ASTRO | n.s. | 0.010 | n.s. | 0.048 | n.s. | 0.020 | ||||||
| OLIGO vs ASTRO | 0.048 | n.s. | ||||||||||
| CTRL vs LOW GRADE | n.s. | 0.008 | n.s. | 0.001 | n.s. | 0.001 | 0.049 | 0.003 | ||||
| LOW vs HIGH GRADE | n.s. | 0.004 | n.s. | 0.018 | n.s. | 0.049 | ||||||
| TUMORS vs CTRL | n.s. | 0.0004 | 0.017 | 0.002 | 0.005 | 0.012 | ||||||