Fig. 3.

SCD1 is involved in the effect of metformin on decreasing TG accumulation in HepG2 cells exposed to high glucose and high insulin. a and b Silencing of SCD1 by shRNA in AML12 cells exposed to high glucose and high insulin simulates the effect of metformin on decreasing TG levelin AML12 cells exposed to high glucose and high insulin. The data are presented as the means ± SEM, n = 4. ^**p < 0.01, versus NC; ^##p < 0.01, versus HGHIn. c and d Overexpression of SCD1 attenuates the effect of metformin on decreasing TG accumulation in HepG2 cells exposed to high glucose and high insulin. The data are presented as the means ± SEM, n = 4. ^**p < 0.01, versus NC; ^#p < 0.05, versus HGHIn. NC normal control, HGHIn high glucose high insulin; Met metformin